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8th NIC@IIT Advanced Microscopy practical workshop

November 27 - December 1, 2023 at

try the new Nikon NSPARC!


  • Paolo Bianchini
  • Alberto Diaspro
Istituto Italiano di Tecnologia, Genova, Italy

Organizing committee

  • Paolo Bianchini
  • Alberto Diaspro
  • Manuela Salvatori

Link to all NIC@IIT Events

Supported by


PNRR Potenziamento Infrastrutture IR00023 SEE LIFE - Potenziamento Euro-BioImaging ERIC Italia

A limited number of students (approx. 40) will be admitted in order to have no more than 5 students for each instrument or set-up.

On November 27th, Lectures will be open access starting from 2,00 pm and ending at 7,00 pm. All together Farwell for invited speakers, students and instructors will be offered at 7,30 pm.

Topics discussed: Sample Preparation; Confocal Microscopy, Image Scanning Microscopy (ISM); STED; STORM, d-STORM; Live Cell Imaging; 2PEFM; FLIM; FCS; Fast Volumetric Imaging; Expansion Microscopy. Starting from November 28th we will have tutorials and practical sessions.

Genoa, Italy

6th NIC@IIT school

Keynote Speakers and Lecturers

  • Martin Oheim

  • Irene Farabella

  • Dario Polli

  • Adi Salomon

  • Pietro Delcanale

  • Francesca Cella Zanacchi

  • Ranieri Bizzarri

  • Giuseppe Vicidomini

  • Peter Saggau

  • Alberto Diaspro

  • Paolo Bianchini

  • Marco Castello

  • Simonluca Piazza

  • Davide Odino

  • Giacomo Cozzi

  • Matteo Parri

  • Mattia Pesce

  • Eleonora Perego

  • Eli Slenders

  • Alessandro Zunino

  • Marcus Oliver Held

  • Sabrina Zappone

  • Andrea Bucci

  • Giacomo Garrè

  • Marco Scotto D'Abbusco

  • Francesca Baldini

  • Lisa Cuneo

  • Fabio Callegari

  • Chantal Usai

  • Matteo Mariangeli

  • Lama Zeaiter

  • Simone Civita

  • Michele Oneto


We would like to thank our partners for their continued support.

To inquire about becoming a partner please contact us.


For registration before performing the payment (link below) contact us:  email This email address is being protected from spambots. You need JavaScript enabled to view it. and This email address is being protected from spambots. You need JavaScript enabled to view it. with the mandatory subject "YOURSURNAME-8TH NIC at IIT-PRE". 

for the PAYMENT click here.

PhD students and junior postdocs: 170€
Postdocs: 250€
groups (more than one person from the same research group): 120€ each person (both PhD students and postdocs)

Companies: 600 €
Non member:
PhD students and junior postdocs: 220 €
Postdocs: 300 €
before proceeding with the payment please contact us

The fee will include technical and scientific material, all together farewell buffet and lunches.

Topics and Workstations

The hands-on session will be organized to give basic knowledge on the following topics and the available instruments. The attendee will be divided into groups of a maximum of three people, and they will turn on all the workstations. While registering, please communicate which is your 2 or 3 most interesting workstations to learn about.


  • N1: Nikon confocal AX NSPARC resonant
    Matteo Parri and Mattia Pesce

  • N2: Nikon N-STORM and N-SIM
    Simone Civita, Lisa Cuneo, and Ranieri Bizzarri

  • N3: Nikon A1MP spectral resonant and FLIM (by ISS)
    Marco Scotto D'Abbusco and Michele Oneto

  • N4: Nikon - CREST spinning disk V3
    Giacomo Cozzi

  • N5Sample Preparation and Expansion Microscopy
    Francesca Baldini, Chantal Usai and Lama Zeaiter

  • N6: Correlative fluorescence-atomic force microscopy
    Matteo Mariangeli and Davide Odino

  • N7: Custom ISM and spad-FFS
    Eli Slenders, Eleonora Perego and Andrea Bucci

  • N8: Custom STED-ISM, FLISM
    Alessandro Zunino, Marcus Oliver Held and Sabrina Zappone

  • N9: Genoa Instruments PRISM
    Marco Castello, Simonluca Piazza and Paolo Bianchini

  • N10: Polarization microscopy, Lab tour and Q&A
    Fabio Callegari, Alberto Diaspro, and Peter Saggau


  • STORM (Stochastic Optical Reconstruction Microscopy), direct-STORM

  • SIM (Structured Illumination Microscopy)

  • Confocal Microscopy

  • ISM (Image Scanning Microscopy)

  • 2PEFM (Two-photon excitation fluorescence microscopy)

  • SHG (Second Harmonic Generation) microscopy

  • STED (STimulated Emission Depletion) microscopy

  • FLIM (Fuorescence Lifetime Imaging Microscopy) and FCS (Fluorescence correlation spectroscopy)

  • Expansion microscopy
  • Polarization microscopy

  • Label-Free microscopy

  • Correlative microscopy

Day one, November 27

Keynote Lectures

  • Monday 27th November, 14.00 to 19.00
  • Location: Palazzo Tobia Pallavicino, Via Garibaldi 4, 16121 Genova


1345 - 14:00


14:00 - 14:05
Welcome and Opening remarks
14:05 - 14:45

Martin Oheim
Université Paris Cité, CNRS, Saints-Pères Paris Institute for the Neurosciences, Paris, France

Fluorescence and SHG imaging with a single-spinning disk two-photon excitation microscope
14:45 - 15:25

Dario Polli
Department of Physics, Politecnico di Milano, Milan, Italy

Vibrational microscopy for label-free chemical identification of molecules
15:25 - 16:05

Adi Salomon
Department of Chemistry, Institute of Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan, Israel

A Colour-Encoded Nanometric Ruler for Axial Super-Resolution Microscopies
16.05 - 16:30

coffee break

16.30 - 17:10

Irene Farabella
Integrative Nuclear Architecture, Istituto Italiano di Tecnologia, Genova, Italy

Investigating 3D genome organisation at the nanoscale with Seq-OligoSTORM
17.10 - 17:50

Pietro Delcanale
Dipartimento di Scienze Matematiche, Fisiche e Informatiche, Università di Parma, Parma, Italy

Single-Molecule Imaging with Reversible Binding Probes
17.50 - 18:30

Francesca Cella Zanacchi
Physics Department E. Fermi, University of Pisa, Pisa

Quantitative insights and molecular determinants mapping by single molecule localization microscopy
18.30 - 19:10

Ranieri Bizzarri
Department of Surgical, Medical and Molecular Pathology, and Critical Care Medicine, University of Pisa, Pisa

The fair switch project: how single molecules reveal the nanoscale of the cell
19.10 - 19:15

Paolo Bianchini & Alberto Diaspro
Nanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Genova Italy

Closing remarks
19.15 - 22:00

Farewell and social dinner

ONLY for registered students and speakers

Day two, November 28

  • Tuesday 28th November to Friday 1st December
  • Closed session for registered students.
  • Location: Istituto Italiano di Tecnologia, via E. Melen 83B (Great Campus Erzelli), Genova


Time10th Floor, Pitagora/Fibonacci meeting room and virtual
10:00 - 10.40

Peter Saggau, Baylor College of Medicine, USA
Acousto-optic devices in Microscopy

10.40 - 11.20

Giuseppe Vicidomini, Istituto Italiano di Tecnologia, Italy
Super-resolution laser scanning microscopy


Groups organization and guide to the hands-on session.

Group Picture at NIC@IIT 

12.30 - 14.00



Microscopy Labs, 9th floor

The students will be divided into groups (G#). All the groups will attend each workstation (N#) for at least an hour. After a complete turn, each group will focus on a subset of methods.
  • G#
    Group number
  • N#
    Workstation number
  G1 G2 G3 G4 G5 G6 G7 G8
 14:15 - 15:15 N1 N2 N3 N4 N5 N6 N7 N8
 15:20 - 16:20 N10 N3 N2 N5 N1 N7 N6 N9
 16:40 - 17:40 N3 N4 N1 N9 N7 N8 N5 N10
 17:45 - 18:45 N4 N1 N6 N3 N8 N9 N10 N2

Day three, November 29

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8
 9:30 - 10:30 N5 N6 N7 N8 N9 N1 N4 N2
 10:40 - 11:40 N6 N7 N8 N10 N9 N2 N1 N3
 11:45 - 12:45 N8 N9 N10 N1 N3 N5 N2 N4
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 15:15 N2 N8 N5 N1 N4 N7 N10 N6
15:20 - 16:20 N6 N2 N1 N8 N5 N3 N4 N7
16:40 - 17:40 N7 N1 N2 N4 N6 N10 N5 N8
17:45 - 18:45 N2 N1 N3 N5 N7 N8 N6 N10

Day four, November 30

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8  
 9:30 - 10:30 N1 N5 N10 N7 N8 N4 N9 N6
 10:45 - 12:45 N10 N8 N9 N3 N1 N5 N6 N2
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 16:15 N9 N7 N4 N6 N2 N10 N3 N5
16:40 - 18:40 N4 N6 N3 N7 N10 N2 N8 N5

Day five, December 1

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8  
 9:30 - 10:30 N6 N4 N8 N2 N9 N1 N5 N7
 10:45 - 12:45 N3 N10 N7 N2 N6 N9 N1 N4