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9th NIC@IIT and MOSBRI Advanced Microscopy practical workshop

December 2 - 6, 2024 at nic.iit.it

Info

Directors

  • Paolo Bianchini
  • Claudio Canale
  • Alberto Diaspro

Istituto Italiano di Tecnologia, Genoa, Italy

Department of Physics, University of Genoa, Genoa, Italy

Organizing committee

  • Paolo Bianchini
  • Claudio Canale
  • Alberto Diaspro
  • Manuela Salvatori

Link to all NIC@IIT Events

Link to all NIC@IIT Events

Supported by

IIT, UNIGE, MOSBRI, SIOF, SIBPA, Nikon

MOSBRI,
European Union’s Horizon 2020 research and innovation programme under grant agreement No 101004806
SEELIFE,
PNRR Potenziamento Infrastrutture IR00023 - Potenziamento Euro-BioImaging ERIC Italia

A limited number of students (approx. 40) will be admitted in order to have no more than 5 students for each instrument or set-up.

On December 2nd, Lectures will be open access starting from 2,00 pm and ending at 7,00 pm. All together Farwell for invited speakers, students and instructors will be offered at 7,30 pm.

Topics discussed: Sample Preparation; Atomic Force Microscopy and spectroscopy; Confocal Microscopy, Image Scanning Microscopy (ISM); STED; STORM, d-STORM; Live Cell Imaging; 2PEFM; FLIM; FCS; Fast Volumetric Imaging; Expansion Microscopy. Starting from December 3rd we will have tutorials and practical sessions.

MOSBRI supports attending this workshop, for participants coming from places where such training is not available locally or in close proximity. 10 selected participants will receive supporting bursaries to attend the course. The bursary will include the registration and 4 nights of accommodation with breakfast (accommodation will be booked by the organizers). To apply for the bursary, please follow the instructions on the MOSBRI website. Application for boursaries is now closed. 

Registration to the workshop is still open.

6th NIC@IIT school

Keynote Speakers and Lecturers

  • Valentina Štimac
  • Maddalena Collini

  • Damian Edward Dalle Nogare

  • Elisa Longo

  • Irene Farabella

  • Francesca Cella Zanacchi

  • Ranieri Bizzarri

  • Giuseppe Vicidomini

  • Peter Saggau

  • Alberto Diaspro

  • Paolo Bianchini

  • Marco Castello

  • Simonluca Piazza

  • Davide Odino

  • Giacomo Cozzi

  • Matteo Parri

  • Eli Slenders

  • Alessandro Zunino

  • Sabrina Zappone

  • Andrea Bucci

  • Giacomo Garrè

  • Marco Scotto D'Abbusco

  • Francesca Baldini

  • Chantal Usai

  • Matteo Mariangeli

  • Lama Zeaiter

  • Simone Civita

  • Michele Oneto

Partners

We would like to thank our partners for their continued support.

MOlecular-Scale Biophysics Research Infrastructure (MOSBRI)
Eurobioimaging

To inquire about becoming a partner please contact us.

Contact us

Registration

For registration before performing the payment, contact us: by email at Manuela Salvatori , Paolo Bianchini and Claudio Canale with the mandatory subject "YOURSURNAME-9TH NIC at IIT-PRE". 

for the PAYMENT please follow the link

For IIT/MOSBRI/UNIGE/SIOF/SIBPA members
PhD students and junior postdocs: 190€
Postdocs: 270€
groups (more than one person from the same research group): 150€ each person (both PhD students and postdocs)

Companies: 600 €
Non-member:
PhD students and junior postdocs: 250 €
Postdocs: 320 €
before proceeding with the payment, please contact us

The fee will include technical and scientific material, all together farewell buffet and lunches.

Topics and Workstations

The hands-on session will be organized to give basic knowledge on the following topics and the available instruments. The attendee will be divided into groups of a maximum of three people, and they will turn on all the workstations. While registering, please communicate which is your 2 or 3 most interesting workstations to learn about.

Workstations

  • N1: Nikon confocal AX NSPARC resonant
    Matteo Parri and Mattia Pesce

  • N2: Nikon N-STORM and N-SIM
    Simone Civita, Lisa Cuneo, and Ranieri Bizzarri

  • N3: Nikon A1MP spectral resonant and FLIM (by ISS)
    Marco Scotto D'Abbusco and Michele Oneto

  • N4: Nikon - CREST spinning disk V3
    Giacomo Cozzi

  • N5: Sample Preparation and Expansion Microscopy
    Francesca Baldini, Chantal Usai and Lama Zeaiter

  • N6: Correlative fluorescence-atomic force microscopy
    Matteo Mariangeli and Davide Odino

  • N7: Custom ISM and spad-FFS
    Eli Slenders, Eleonora Perego and Andrea Bucci

  • N8: Custom STED-ISM, FLISM
    Alessandro Zunino, Marcus Oliver Held and Sabrina Zappone

  • N9: Genoa Instruments PRISM
    Marco Castello, Simonluca Piazza and Paolo Bianchini

  • N10: Polarization microscopy, Lab tour and Q&A
    Fabio Callegari, Alberto Diaspro, and Peter Saggau

Topics

  • AFM (Atomic Force Microscopy)

  • STORM (Stochastic Optical Reconstruction Microscopy), direct-STORM

  • SIM (Structured Illumination Microscopy)

  • Confocal Microscopy

  • ISM (Image Scanning Microscopy)

  • 2PEFM (Two-photon excitation fluorescence microscopy)

  • SHG (Second Harmonic Generation) microscopy

  • STED (STimulated Emission Depletion) microscopy

  • FLIM (Fuorescence Lifetime Imaging Microscopy) and FCS (Fluorescence correlation spectroscopy)

  • Expansion microscopy

  • Polarization microscopy

  • Label-Free microscopy

  • Correlative microscopy

Day one, December 2

Keynote Lectures

  • Monday 2nd December, 14.00 to 19.00
  • Location: Palazzo Tobia Pallavicino, Via Garibaldi 4, 16121 Genova

Program

TimeSpeaker/sTitle
13:45 - 14:00

Registration

14:00 - 14:05
Welcome and Opening remarks
14:05 - 14:45

Valentina Štimac

Super-resolution imaging of mitotic spindle microtubules using STED microscopy
14:45 - 15:25

Damian Edward Dalle Nogare

Bioimage analysis in the age of artificial intelligence
15:25 - 16:05

Maddalena Collini

Implantable micro-optics for label-free non-linear imaging
16.05 - 16:30

coffee break

16.30 - 17:10

Elisa Longo

Segmented FCS on a commercial microscope: a tool for studying dynamics and interactions in live cells
17.10 - 17:50

Ranieri Bizzarri

The conceptual basis of single molecule imaging (with some examples)
17.50 - 18:30

Francesca Cella Zanacchi

Advanced single-molecule super-resolution and its applications
18.30 - 18:45

Paolo Bianchini, Claudio Canale and Alberto Diaspro
Nanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Genoa Italy
Department of Physics, University of Genoa, Genoa, Italy

Closing remarks
19.00 - 21:00

Farewell with drinks and nibbles

ONLY for registered students and speakers

Day two, December 3

  • Tuesday 3rd December to Friday 6th December
  • Closed session for registered students.
  • Location: Istituto Italiano di Tecnologia, via E. Melen 83B (Great Campus Erzelli), Genova

Program

Time10th Floor, Pitagora/Fibonacci meeting room and virtual
09:50-10:00

Groups organization and guide to the hands-on session.

10.00 - 10.40

Irene Farabella, Istituto Italiano di Tecnologia, Italy

10:40 - 11.20

Peter Saggau, Baylor College of Medicine, USA
Acousto-optic devices in Microscopy

11.20 - 12.00

Giuseppe Vicidomini, Istituto Italiano di Tecnologia, Italy
Super-resolution laser scanning microscopy with detector array

12.00 - 12.40

Claudio Canale, University of Genoa, Italy
Exploring Intermolecular Processes with Correlative AFM-STED Microscopy

12.40 - 14.00

Group picture and Lunch

Hands-on

Microscopy Labs, 9th floor

The students will be divided into groups (G#). All the groups will attend each workstation (N#) for at least an hour. After a complete turn, each group will focus on a subset of methods.
  • G#
    Group number
  • N#
    Workstation number
  G1 G2 G3 G4 G5 G6 G7 G8
 14:15 - 15:15 N1 N2 N3 N4 N5 N6 N7 N8
 15:20 - 16:20 N10 N3 N2 N5 N1 N7 N6 N9
 16:40 - 17:40 N3 N4 N1 N9 N7 N8 N5 N10
 17:45 - 18:45 N4 N1 N6 N3 N8 N9 N10 N2

Day three, December 4

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8
 9:30 - 10:30 N5 N6 N7 N8 N9 N1 N4 N2
 10:40 - 11:40 N6 N7 N8 N10 N9 N2 N1 N3
 11:45 - 12:45 N8 N9 N10 N1 N3 N5 N2 N4
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 15:15 N2 N8 N5 N1 N4 N7 N10 N6
15:20 - 16:20 N6 N2 N1 N8 N5 N3 N4 N7
16:40 - 17:40 N7 N1 N2 N4 N6 N10 N5 N8
17:45 - 18:45 N2 N1 N3 N5 N7 N8 N6 N10

Day four, December 5

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8  
 9:30 - 10:30 N1 N5 N10 N7 N8 N4 N9 N6
 10:45 - 12:45 N10 N8 N9 N3 N1 N5 N6 N2
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 16:15 N9 N7 N4 N6 N2 N10 N3 N5
16:40 - 18:40 N4 N6 N3 N7 N10 N2 N8 N5

Day five, December 6

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8  
 9:30 - 10:30 N6 N4 N8 N2 N9 N1 N5 N7
 10:45 - 12:45 N3 N10 N7 N2 N6 N9 N1 N4
  CLOSING REMARKS and lunch

Photogallery

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