9th NIC@IIT and MOSBRI Advanced Microscopy practical workshop
December 2 - 6, 2024 at nic.iit.it
Info
Directors
- Paolo Bianchini
- Claudio Canale
- Alberto Diaspro
Istituto Italiano di Tecnologia, Genoa, Italy
Department of Physics, University of Genoa, Genoa, Italy
Organizing committee
- Paolo Bianchini
- Claudio Canale
- Alberto Diaspro
- Manuela Salvatori
Link to all NIC@IIT Events
Link to all NIC@IIT Events
A limited number of students (approx. 40) will be admitted in order to have no more than 5 students for each instrument or set-up.
On December 2nd, Lectures will be open access starting from 2,00 pm and ending at 7,00 pm. All together Farwell for invited speakers, students and instructors will be offered at 7,30 pm.
Topics discussed: Sample Preparation; Atomic Force Microscopy and spectroscopy; Confocal Microscopy, Image Scanning Microscopy (ISM); STED; STORM, d-STORM; Live Cell Imaging; 2PEFM; FLIM; FCS; Fast Volumetric Imaging; Expansion Microscopy. Starting from December 3rd we will have tutorials and practical sessions.
MOSBRI supports attending this workshop, for participants coming from places where such training is not available locally or in close proximity. 10 selected participants will receive supporting bursaries to attend the course. The bursary will include the registration and 4 nights of accommodation with breakfast (accommodation will be booked by the organizers). To apply for the bursary, please follow the instructions on the MOSBRI website.
Keynote Speakers and Lecturers
- TBA
- TBA
- TBA
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TBA
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TBA
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Francesca Cella Zanacchi
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Ranieri Bizzarri
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Giuseppe Vicidomini
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Peter Saggau
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Alberto Diaspro
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Paolo Bianchini
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Marco Castello
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Simonluca Piazza
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Davide Odino
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Giacomo Cozzi
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Matteo Parri
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Mattia Pesce
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Eli Slenders
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Alessandro Zunino
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Marcus Oliver Held
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Sabrina Zappone
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Andrea Bucci
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Giacomo Garrè
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Marco Scotto D'Abbusco
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Francesca Baldini
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Lisa Cuneo
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Chantal Usai
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Matteo Mariangeli
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Lama Zeaiter
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Simone Civita
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Michele Oneto
Partners
We would like to thank our partners for their continued support.
Registration
For registration before performing the payment, contact us: by email at Manuela Salvatori , Paolo Bianchini and Claudio Canale with the mandatory subject "YOURSURNAME-9TH NIC at IIT-PRE".
PAYMENT instructions will be announced as soon as possible.
The fee will include technical and scientific material, all together farewell buffet and lunches.
Topics and Workstations
Workstations
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N1: Nikon confocal AX NSPARC resonant
Matteo Parri and Mattia Pesce -
N2: Nikon N-STORM and N-SIM
Simone Civita, Lisa Cuneo, and Ranieri Bizzarri -
N3: Nikon A1MP spectral resonant and FLIM (by ISS)
Marco Scotto D'Abbusco and Michele Oneto -
N4: Nikon - CREST spinning disk V3
Giacomo Cozzi -
N5: Sample Preparation and Expansion Microscopy
Francesca Baldini, Chantal Usai and Lama Zeaiter -
N6: Correlative fluorescence-atomic force microscopy
Matteo Mariangeli and Davide Odino -
N7: Custom ISM and spad-FFS
Eli Slenders, Eleonora Perego and Andrea Bucci -
N8: Custom STED-ISM, FLISM
Alessandro Zunino, Marcus Oliver Held and Sabrina Zappone -
N9: Genoa Instruments PRISM
Marco Castello, Simonluca Piazza and Paolo Bianchini -
N10: Polarization microscopy, Lab tour and Q&A
Fabio Callegari, Alberto Diaspro, and Peter Saggau
Topics
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AFM (Atomic Force Microscopy)
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STORM (Stochastic Optical Reconstruction Microscopy), direct-STORM
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SIM (Structured Illumination Microscopy)
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Confocal Microscopy
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ISM (Image Scanning Microscopy)
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2PEFM (Two-photon excitation fluorescence microscopy)
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SHG (Second Harmonic Generation) microscopy
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STED (STimulated Emission Depletion) microscopy
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FLIM (Fuorescence Lifetime Imaging Microscopy) and FCS (Fluorescence correlation spectroscopy)
- Expansion microscopy
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Polarization microscopy
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Label-Free microscopy
- Correlative microscopy
Day one, December 2
Keynote Lectures
- Monday 2nd December, 14.00 to 19.00
- Location: Palazzo Tobia Pallavicino, Via Garibaldi 4, 16121 Genova
Program
Time | Speaker/s | Title |
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1345 - 14:00 | Registrtion | |
14:00 - 14:05 | Welcome and Opening remarks | |
14:05 - 14:45 | TBA | |
14:45 - 15:25 | TBA | |
15:25 - 16:05 | TBA | |
16.05 - 16:30 | coffee break | |
16.30 - 17:10 | TBA | |
17.10 - 17:50 | TBA | |
17.50 - 18:30 | TBA | |
18.30 - 19:10 | TBA | |
19.10 - 19:15 | Paolo Bianchini, Claudio Canale and Alberto Diaspro | Closing remarks |
19.15 - 22:00 | Farewell and social dinner | ONLY for registered students and speakers |
Day two, December 3
- Tuesday 3rd December to Friday 6th December
- Closed session for registered students.
- Location: Istituto Italiano di Tecnologia, via E. Melen 83B (Great Campus Erzelli), Genova
Program
Time | 10th Floor, Pitagora/Fibonacci meeting room and virtual |
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10:00 - 10.40 | Peter Saggau, Baylor College of Medicine, USA |
10.40 - 11.20 | Giuseppe Vicidomini, Istituto Italiano di Tecnologia, Italy |
11.30 | Groups organization and guide to the hands-on session. Group Picture at NIC@IIT |
12.30 - 14.00 | Lunch |
Hands-on
Microscopy Labs, 9th floor
The students will be divided into groups (G#). All the groups will attend each workstation (N#) for at least an hour. After a complete turn, each group will focus on a subset of methods.
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G#Group number
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N#Workstation number
G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | ||
14:15 - 15:15 | N1 | N2 | N3 | N4 | N5 | N6 | N7 | N8 | |
15:20 - 16:20 | N10 | N3 | N2 | N5 | N1 | N7 | N6 | N9 | |
16:40 - 17:40 | N3 | N4 | N1 | N9 | N7 | N8 | N5 | N10 | |
17:45 - 18:45 | N4 | N1 | N6 | N3 | N8 | N9 | N10 | N2 |
Day three, December 4
Hands on
G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | ||
9:30 - 10:30 | N5 | N6 | N7 | N8 | N9 | N1 | N4 | N2 | |
10:40 - 11:40 | N6 | N7 | N8 | N10 | N9 | N2 | N1 | N3 | |
11:45 - 12:45 | N8 | N9 | N10 | N1 | N3 | N5 | N2 | N4 | |
13:00 - 14:00 | LUNCH (1st floor packet lunch reserved area) | ||||||||
14:15 - 15:15 | N2 | N8 | N5 | N1 | N4 | N7 | N10 | N6 | |
15:20 - 16:20 | N6 | N2 | N1 | N8 | N5 | N3 | N4 | N7 | |
16:40 - 17:40 | N7 | N1 | N2 | N4 | N6 | N10 | N5 | N8 | |
17:45 - 18:45 | N2 | N1 | N3 | N5 | N7 | N8 | N6 | N10 |
Day four, December 5
Hands on
G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | ||
9:30 - 10:30 | N1 | N5 | N10 | N7 | N8 | N4 | N9 | N6 | |
10:45 - 12:45 | N10 | N8 | N9 | N3 | N1 | N5 | N6 | N2 | |
13:00 - 14:00 | LUNCH (1st floor packet lunch reserved area) | ||||||||
14:15 - 16:15 | N9 | N7 | N4 | N6 | N2 | N10 | N3 | N5 | |
16:40 - 18:40 | N4 | N6 | N3 | N7 | N10 | N2 | N8 | N5 |