4^th NIC@IIT Advanced Microscopy practical workshop

November 27 - December 1, 2017 at nic.iit.it

Info

Directors

Paolo Bianchini, Alberto Diaspro

Istituto Italiano di Tecnologia, Genova, Italy

A limited number of students (approx. 40) will be admitted in order to have no more than 3/4 students for each instrument or set-up.

On November 27th, Lectures will be open access starting from 2,30 pm ending at 7,30 pm. All together dinner for invited speakers, students and instructors will be offered at 8 pm.

Basic courses will be included for students on:

Confocal, Image scanning microscopy (ISM) and STED; STORM, d-STORM; Live cell imaging; 2-photon and FLIM/FRET; Fast volumetric imaging and particle tracking; Expansion Microscopy.

Starting from November 28th we will have tutorials and practical sessions. On December 1st we will end at lunch time.

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Registration Fees

For registration, visit fill the form at SIOF website or This email address is being protected from spambots. You need JavaScript enabled to view it., in order to book the seat or to apply for bursaries - payments can be done later - please, send an email to "This email address is being protected from spambots. You need JavaScript enabled to view it." and "This email address is being protected from spambots. You need JavaScript enabled to view it." with the mandatory subject "YOURSURNAME-4TH NIC at IIT-PRE".

Type	FEE IN EUROS (€)
PhD student	180€
Post Doc	250€
Professionals or companies	600€
IIT/SIOF/SIBPA/UNIGE members: PhD students and junior Post Doc	120€
IIT/SIOF/SIBPA/UNIGE members: Post Docs	200€
The fee will include technical and scientific material, all together dinner and packet lunch.

Speakers

Monday 27th November, 14.00 to 19.00

Location: Sala delle Grida - City Center - Piazza De Ferrari, Genova

Open access session

14.00	Paolo Bianchini & Alberto Diaspro, Istituto Italiano di Tecnologia, Italy	Welcome and Opening remarks
14.15	Peter Saggau, Allen Institute for Brain Science, USA	Scalable Approaches for Encoded Optical Imaging
14.45	Emiliano Ronzitti, Paris Descartes University, France	Shaping the light for targeted interrogation of neural circuits
15.15	Thomas Huser, University of Bielefeld, Germany	High resolution and high speed imaging of living cells by multimodal optical nanoscopy
15.45	Kseniya Korobchevskaya, Kennedy Inst. of Rheumatology, UK	Structured Illumination microscopy for live super-resolution imaging
16.15	Coffee break
16.45	Matteo Bardelli, Physik Instrumente, Italy	TBD
17.00	Silvia Galiani, University of Oxford, UK	Exploring peroxisomes import process: an advance cellular microscopy study
17.30	Zeno Lavagnino, Washington University, US	diSPIM allows multidimensional characterisation of second messengers activity in intact islets of Langerhans
18.00	Matthieu Dubreuil, University of Brest, France	Polarimetric methods for linear and nonlinear microscopy
18.30	Alberto Diaspro, Istituto Italiano di Tecnologia, Italy	The Extraordinary Microscope
20.00	Social Dinner	For registered students and speakers

Program

Tutorials and Practical workshop

Please note that this is a closed session for registered students.

Each student should choose his favorite course and the corresponding group. We will try to satisfy all the requests. However, the maximum number of students per group is 4. Once assigned to a group, students cannot exchange them. Each group will attend eight "hands on" lessons in eight different instruments as shown in the programme.

Tutorials

Time lapse (widefield and TIRF), JOBS Mattia Pesce
Spinning disk / Confocal, ISM Giuseppe Vicidomini
Multiphoton, FLIM, FRET Luca Lanzanò
STED, STED-FCS Silvia Galiani
STORM, PALM, GSDIM Francesca Cella Zanacchi
SIM Kseniya Korobchevskaya
Fast volumetric imaging Marti Duocastella
Sample preparation for super-resolution microscopy Luca Pesce

Courses

Course	Suggested Tutorials	Hands on (at least)	Group number
Confocal, ISM and STED	b., d., h.	N3, N5, N6, N9, N10	G1, G2
STORM, d-STORM	a., e., h.	N1, N2, N5, N7, N8	G3, G4
Live cell imaging	a., f., h.	N2, N4, N5, N7, N8	G5
2-photon and FLIM/FRET	b., d., c., h.	N2, N3, N5, N6, N10	G6
Fast volumetric imaging and particle tracking.	a., c., g.	N1, N2, N7, N5, N11	G7
Expansion Microscopy	b., d. f., h.	N4, N8, N9, N5, N12	G8

Instruments and workstations

N1: Nikon multicolor 3D N-STORM
N2: IIT custom SPIM, IML-SPIM
N3: IIT custom gSTED, CARMA-Gi
N4: Nikon N-SIM
N5: Nikon A1 spectral confocal
N6: Nikon Time lapse

N7: Fast Confocal Nikon spinning disk
N8: IIT custom 2C 3D pulsed STED
N9: Nikon A1R MP, ISS fast FLIM and FCS
N10: single particle tracking
N11: Expansion Microscopy

Tuesday 28

Time	HACK room, IIT, Genova, Italy	MONTALCINI room, IIT, Genova, Italy
09.30 – 10.30	a. (Time lapse)	b. (confocal)
10.30 – 11.30	e. (STORM)	d. (STED)
11.30 – 12.30	f. (SIM)	c. (FLIM, FRET)
12.30 – 14.00	LUNCH
14.00 – 15.00	g. (Fast volumetric imagingSample preparation)