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10th NIC@IIT Advanced Microscopy practical workshop

December 1- 5, 2025 at nic.iit.it

Info

Directors

  • Paolo Bianchini
  • Alberto Diaspro

Istituto Italiano di Tecnologia, Genoa, Italy

Department of Physics, University of Genoa, Genoa, Italy

Organizing committee

  • Paolo Bianchini
  • Alberto Diaspro
  • Manuela Salvatori

Link to all NIC@IIT Events

Link to all NIC@IIT Events

Supported by

IIT, UNIGE, SIOF, SIBPA, Nikon

SEELIFE,
PNRR Potenziamento Infrastrutture IR00023 - Potenziamento Euro-BioImaging ERIC Italia

A limited number of students (approx. 40) will be admitted in order to have no more than 5 students for each instrument or set-up.

On December 1st, Lectures will be open access starting from 2,00 pm and ending at 7,00 pm. All together Farwell for invited speakers, students and instructors will be offered at 7,30 pm.

Topics discussed: Sample Preparation; Atomic Force Microscopy and spectroscopy; Confocal Microscopy, Image Scanning Microscopy (ISM); STED; STORM, d-STORM; Live Cell Imaging; 2PEFM; FLIM; FCS; Fast Volumetric Imaging; Expansion Microscopy. Starting from December 2nd we will have tutorials and practical sessions.

6th NIC@IIT school

Keynote Speakers and Lecturers

  • Melike Lakadamyali
  • Takahiro Deguchi

  • Stefan Stanciu

  • Francesca Cella Zanacchi

  • Ranieri Bizzarri

  • Giuseppe Vicidomini

  • Peter Saggau

  • Alberto Diaspro

  • Paolo Bianchini

  • Marco Castello

  • Simonluca Piazza

  • Davide Odino

  • Giacomo Cozzi

  • Matteo Parri

  • Claudio Canale

  • Gaytri Arya

  • Eli Slenders

  • Andrea Bucci

  • Giacomo Garrè

  • Marco Scotto D'Abbusco

  • Chantal Usai

  • Matteo Mariangeli

  • Martina Simeone

  • Michele Oneto

Partners

We would like to thank our partners for their continued support.

MOlecular-Scale Biophysics Research Infrastructure (MOSBRI)
Eurobioimaging

To inquire about becoming a partner please contact us.

Contact us

Registration

For registration before performing the payment, contact us: by email at Manuela Salvatori , Paolo Bianchini with the mandatory subject "YOURSURNAME-10TH NIC at IIT-PRE". 

for the PAYMENT please wait. The instructions will be available soon on this webpage.

For IIT/MOSBRI/UNIGE/SIOF/SIBPA members
PhD students and junior postdocs: 190€
Postdocs: 270€
groups (more than one person from the same research group): 150€ each person (both PhD students and postdocs)

Companies: 600 €
Non-member:
PhD students and junior postdocs: 250 €
Postdocs: 320 €
before proceeding with the payment, please contact us

The fee will include technical and scientific material, all together farewell buffet and lunches.

Topics and Workstations

The hands-on session will be organized to give basic knowledge on the following topics and the available instruments. The attendee will be divided into groups of a maximum of three people, and they will turn on all the workstations. While registering, please communicate which is your 2 or 3 most interesting workstations to learn about.

Workstations

  • N1: Nikon confocal AX NSPARC resonant
    Mattia Pesce

  • N2: Nikon N-STORM and N-SIM
    Chantal Usai, Alberto Morgantini, Vittorio Parenti

  • N3: Nikon A1MP spectral resonant and FLIM (by ISS)
    Marco Scotto D'Abbusco and Mattia Donato

  • N4: Nikon - CREST spinning disk V3
    Giacomo Cozzi and Matteo Parri

  • N5: Sample Preparation and Expansion Microscopy
    Michele Oneto, Francesca Baldini, and Lama Zeaiter

  • N6: Correlative fluorescence-atomic force microscopy
    Matteo Mariangeli, Davide Odino, and Claudio Canale

  • N7: Custom ISM for spectroscopy
    Eli Slenders, Simone Civita and Andrea Bucci, Sanket Patil

  • N8: Custom ISM for Imaging
    Alessandro Zunino, Francesco Del Bufalo, Giacomo Garrè, Lisa Cuneo

  • N9: Genoa Instruments PRISM
    Marco Castello, Simonluca Piazza and Paolo Bianchini

  • N10: Optics, label-free and quantum Imaging, Lab tour, Q&A
    Gaytri Arya, Nicolò Incardona, Alberto Diaspro, and Peter Saggau

Topics

  • AFM (Atomic Force Microscopy)

  • STORM (Stochastic Optical Reconstruction Microscopy), direct-STORM

  • SIM (Structured Illumination Microscopy)

  • Confocal Microscopy

  • ISM (Image Scanning Microscopy)

  • 2PEFM (Two-photon excitation fluorescence microscopy)

  • SHG (Second Harmonic Generation) microscopy

  • STED (STimulated Emission Depletion) microscopy

  • FLIM (Fuorescence Lifetime Imaging Microscopy) and FCS (Fluorescence correlation spectroscopy)

  • Expansion microscopy

  • Polarization microscopy

  • Label-Free microscopy

  • Correlative microscopy

Day one, December 1

Keynote Lectures

  • Monday 1st December, 14.00 to 19.00
  • Location: Palazzo Tobia Pallavicino, Via Garibaldi 4, 16121 Genova

Program

TimeSpeaker/sTitle
13:45 - 14:00

Registration

14:00 - 14:05
Welcome and Opening remarks
14:05 - 14:45

Melike Lakadamyali

TBD
14:45 - 15:25

Takahiro Deguchi

TBD
15:25 - 16:05
16.05 - 16:40

coffee break

16:40 - 17:10

Ranieri Bizzarri

The conceptual basis of single molecule imaging (with some examples)
17.10 - 17:50

Francesca Cella Zanacchi

Advanced single-molecule super-resolution and its applications
17.50 - 18:15

Paolo Bianchini, and Alberto Diaspro
Nanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Genoa Italy
Department of Physics, University of Genoa, Genoa, Italy

Closing remarks
18.30 - 20:00

Farewell with drinks and nibbles

ONLY for registered students and speakers

Day two, December 2

  • Tuesday 2nd December to Friday 6th December
  • Closed session for registered students.
  • Location: Istituto Italiano di Tecnologia, via E. Melen 83B (Great Campus Erzelli), Genova

Program

Time10th Floor, Pitagora/Fibonacci meeting room and virtual
09:50-10:30

Irene Farabella, Istituto Italiano di Tecnologia, Italy
Investigating 3D genome  organisation at the nanoscale with Seq-OligoSTORM

10.30 - 11.10

Giuseppe Vicidomini, Istituto Italiano di Tecnologia, Italy
Super-resolution laser scanning microscopy with detector array

11:10 - 11.50

Peter Saggau, Baylor College of Medicine, USA
Acousto-optic devices in Microscopy

12.00 - 12.40

Claudio Canale, University of Genoa, Italy
Exploring Intermolecular Processes with Correlative AFM-STED Microscopy

12.40 - 14.00

Group picture and Lunch

Hands-on

Microscopy Labs, 9th floor

The students will be divided into groups (G#). All the groups will attend each workstation (N#) for at least an hour. After a complete turn, each group will focus on a subset of methods.
  • G#
    Group number
  • N#
    Workstation number
  G1 G2 G3 G4 G5 G6 G7
 14:15 - 15:15 N1 N2 N3 N4 N6 N7 N8
 15:20 - 16:20 N10 N3 N2 N5 N7 N6 N9
 16:40 - 17:40 N3 N4 N1 N9 N8 N5 N10
 17:45 - 18:45 N4 N1 N6 N3 N9 N10 N2

Day three, December 3

Hands on

  G1  G2  G3  G4    G5  G6   G7
 9:30 - 10:30 N5 N6 N7 N8 N1 N4 N2
 10:40 - 11:40 N6 N7 N8 N10 N2 N1 N3
 11:45 - 12:45 N8 N9 N10 N1 N5 N2 N4
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 15:15 N2 N8 N5 N1 N7 N10 N6
15:20 - 16:20 N6 N2 N1 N8 N3 N4 N7
16:40 - 17:40 N7 N1 N2 N4 N10 N5 N8
17:45 - 18:45 N2 N1 N3 N5 N8 N6 N10

Day four, December 4

Hands on

  G1  G2  G3  G4  G5  G6   G7  
 9:30 - 10:30 N1 N5 N10 N7 N4 N9 N6
 10:45 - 12:45 N10 N8 N9 N3 N5 N6 N2
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 16:15 N9 N7 N4 N6 N10 N3 N5
16:40 - 18:40 N4 N6 N3 N7 N2 N8 N5

Day five, December 5

Hands on

  G1  G2  G3  G4  G5  G6   G7  
 9:30 - 10:30 N6 N4 N8 N2 N1 N5 N7
 10:45 - 12:45 N3 N10 N7 N2 N9 N1 N4
  CLOSING REMARKS and lunch

Photogallery

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