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10th NIC@IIT Advanced Microscopy practical workshop

December 1- 5, 2025 at nic.iit.it

Info

download the full schedule

Directors

  • Paolo Bianchini
  • Alberto Diaspro

Istituto Italiano di Tecnologia, Genoa, Italy

Department of Physics, University of Genoa, Genoa, Italy

Organizing committee

  • Paolo Bianchini
  • Alberto Diaspro
  • Manuela Salvatori

Link to all NIC@IIT Events

Link to all NIC@IIT Events

Supported by

IIT, UNIGE, SIOF, SIBPA, Nikon

SEELIFE,
PNRR Potenziamento Infrastrutture IR00023 - Potenziamento Euro-BioImaging ERIC Italia

A limited number of students (approx. 30) will be admitted in order to have no more than 5 students for each instrument or set-up.

On December 1st, Lectures will be open access starting from 2,00 pm and ending at 7,00 pm. All together Farwell for invited speakers, students and instructors will be offered at 7,30 pm.

Topics discussed: Sample Preparation; Atomic Force Microscopy and spectroscopy; Confocal Microscopy, Image Scanning Microscopy (ISM); STED; STORM, d-STORM; Live Cell Imaging; 2PEFM; FLIM; FCS; Fast Volumetric Imaging; Expansion Microscopy. Starting from December 2nd we will have tutorials and practical sessions.

6th NIC@IIT school

Keynote Speakers and Lecturers

  • Melike Lakadamyali
  • Takahiro Deguchi
  • Vittoria Raffa
  • Ryu Nakamura

  • Irene Farabella

  • Francesca Cella Zanacchi

  • Ranieri Bizzarri

  • Giuseppe Vicidomini

  • Peter Saggau

  • Alberto Diaspro

  • Paolo Bianchini

  • Marco Castello

  • Simonluca Piazza

  • Federica Quici

  • Gioele Figus

  • Nicolo Incardona

  • Larbi Touijer

  • Giacomo Cozzi

  • Matteo Parri

  • Chantal Usai

  • Gaytri Arya

  • Eli Slenders

  • Andrea Bucci

  • Laurent Le

  • Sanket Patil

  • Giacomo Garrè

  • Lisa Cuneo

  • Mattia Donato

  • Mattia Pesce

  • Claudio Canale

  • Paolo Canepa

  • Andrea Lagomarsino

  • Matteo Mariangeli

  • Martina Simeone

  • Vittorio Parenti

  • Michele Oneto

  • Marco Scotto D'Abbusco

Partners

We would like to thank our partners for their continued support.

MOlecular-Scale Biophysics Research Infrastructure (MOSBRI)
Eurobioimaging

To inquire about becoming a partner please contact us.

Contact us

Registration

For registration before performing the payment, contact us: by email at Manuela Salvatori , Paolo Bianchini with the mandatory subject "YOURSURNAME-10TH NIC at IIT-PRE". 

for the PAYMENT please follow this link. However, before proceeding with the payment, please contact us.

For IIT/MOSBRI/UNIGE/SIOF/SIBPA members
PhD students and junior postdocs: 190€
Postdocs: 270€
groups (more than one person from the same research group): 150€ each person (both PhD students and postdocs)

Companies: 600 €
Non-member:
PhD students and junior postdocs: 250 €
Postdocs: 320 €
before proceeding with the payment, please contact us

The fee will include technical and scientific material, all together farewell buffet and lunches.

Topics and Workstations

The hands-on sessions are designed to provide participants with foundational knowledge of the main topics covered in the school and to introduce the available instruments. Attendees will be divided into small groups of up to five participants and will rotate across all workstations. During registration, please indicate your preferred group or 2–3 preferred workstations so we can take your interests into account.
After completing a full rotation, each group will participate in an in-depth session on three selected instruments:

Group 1: N2, N3, N4 Group 2: N3, N4, N5 Group 3: N1, N4, N9 Group 4: N5, N6, N7 Group 5: N1, N2, N9

Workstations

  • N1: Nikon confocal AX NSPARC resonant
    Ryu Nakamura, Paolo Bianchini, Mattia Pesce

  • N2: Nikon N-STORM
    Martina Simeone, Chantal Usai, Vittorio Parenti

  • N3: Nikon A1MP spectral resonant and FLIM (by ISS)
    Marco Scotto D'Abbusco and Mattia Donato

  • N4: Nikon - JI
    Giacomo Cozzi and Matteo Parri

  • N5: Genoa Instruments PRISM
    Marco Castello, Federica Quici, Gioele Figus

  • N6: Correlative fluorescence-atomic force microscopy
    Paolo Canepa, Andrea Lagomarsino, Claudio Canale

  • N7: Custom ISM for microscopy and spectroscopy
    E. Slenders, L. Le, A. Bucci, G.Garrè, L. Cuneo

  • N8: Sample Preparation and Expansion Microscopy
    Michele Oneto, Hawraa Zbeeb

  • N9: Optics, label-free and quantum Imaging, Lab tour, Q&A
    Nicolò Incardona, Gaytri Arya, Larbi Touijer, and Peter Saggau

Topics

  • MINFLUX (minimal fluorescence photon fluxes microscopy)

  • AFM (Atomic Force Microscopy)

  • STORM (Stochastic Optical Reconstruction Microscopy)

  • SMLM (Single Molecule Localization Microscopy)

  • Confocal Laser Scanning Fluorescence Microscopy

  • ISM (Image Scanning Microscopy)

  • 2PEFM (Two-photon excitation fluorescence microscopy)

  • SHG (Second Harmonic Generation) microscopy

  • STED (STimulated Emission Depletion) microscopy

  • FLIM (Fuorescence Lifetime Imaging Microscopy)

  • FCS (Fluorescence correlation spectroscopy)

  • Expansion microscopy

  • Polarization microscopy

  • Label-Free microscopy

  • Correlative microscopy

Day one, December 1

Keynote Lectures

  • Monday 1st December, 14.00 to 19.00
  • Location: Sala delle Grida,
    Palazzo della Borsa, Via XX Settembre 44, 16121 Genova

Program

TimeSpeaker/sTitle
13:45 - 14:00

Registration

14:00 - 14:05
Welcome and Opening remarks
14:05 - 14:45
Melike Lakadamyali
Department of Physiology, University of Pennsylvania, USA
Super-resolution imaging of chromatin in health and disease
14:45 - 15:25

Takahiro Deguchi

EMBL Imaging Centre, EMBL-Heidelberg , Heidelberg, Germany
Single-Molecule Localization and MINFLUX Microscopy: From Static Structures to Dynamic Protein Function
15:25 - 16:05

Ryu Nakamura
System Development Dept., Technology Solution Sector,Healthcare Business Unit, Nikon Corporation

Efficient and quantitative imaging with NSPARC: best practices and case studies
16.05 - 16:30

coffee break

16:30 - 17:10

Ranieri Bizzarri
Department of Surgical, Medical and Molecular Pathology, and Critical Care Medicine, University of Pisa, Pisa, Italy

The conceptual basis of single molecule imaging (with some examples)
17.10 - 17.50

Francesca Cella Zanacchi
Department of Physics, University of Pisa, Pisa, Italy

Advanced single-molecule super-resolution and its applications
17.50 - 18:30

Vittoria Raffa
Dept. of Biology, University of Pisa, Pisa, Italy

Are microtubules the drivers of neuronal mechanotransduction?
18.30 - 19:00

Paolo Bianchini, and Alberto Diaspro
Nanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Genoa Italy
Department of Physics, University of Genoa, Genoa, Italy

Roud Table and closing remarks
19.00 - 20:30

Farewell with drinks and nibbles

ONLY for registered students and speakers

Day two, December 2

  • Tuesday 2nd December to Friday 6th December
  • Closed session for registered students.
  • Location: Istituto Italiano di Tecnologia, via E. Melen 83B (Great Campus Erzelli), Genova

Program

Time10th Floor, Pitagora/Fibonacci meeting room and virtual
09:50-10:30

Irene Farabella, Istituto Italiano di Tecnologia, Italy
Investigating 3D genome  organisation at the nanoscale with Seq-OligoSTORM

10.30 - 11.10

Giuseppe Vicidomini, Istituto Italiano di Tecnologia, Italy
Super-resolution laser scanning microscopy with detector array

11:10 - 11.50

Peter Saggau, Baylor College of Medicine, USA
Acousto-optic devices in Microscopy

11.50 - 12.30

Claudio Canale, University of Genoa, Italy
Exploring Intermolecular Processes with Correlative AFM-STED Microscopy

12.30 - 14.10

Group picture and Lunch

Hands-on

Microscopy Labs, 9th floor

The students will be divided into groups (G#). All the groups will attend each workstation (N#) for at least an hour. After a complete turn, each group will focus on a subset of methods.
  • G#
    Group number
  • N#
    Workstation number
Group 1 Group 2 Group 3 Group 4 Group 5
 14:10 - 16:10 N2 N3 N4 N5 N6
16:10 - 16:40 coffee break
 16:40 - 18:40 N3 N4 N5 N6 N7

Day three, December 3

Hands on

Group 1 Group 2 Group 3 Group 4 Group 5
 9:30 - 11:00 N4 N5 N6 N7 N8
11:00 - 11:20 coffee break
11:20 - 12:50 N5 N6 N7 N8 N9
12:50 - 14:10 LUNCH (1st floor packet lunch reserved area)
 14:10 - 16:10 N6 N7 N8 N9 N1
 16:10 - 16:40 coffee break
 16:40 - 18:40 N7 N8 N9 N1 N2

Day four, December 4

Hands on

Group 1 Group 2 Group 3 Group 4 Group 5
 9:30 - 11:00 N8 N9 N1 N2 N3
11:00 - 11:20 coffee break
11:20 - 12:50 N9 N1 N2 N3 N4
12:50 - 14:10 LUNCH (1st floor packet lunch reserved area)
 14:10 - 16:10 N1 N2 N3 N4 N5
 16:10 - 16:40 coffee break
 16:40 - 18:40 N2 N3 N4 N5 N1

Day five, December 5

Hands on

Group 1 Group 2 Group 3 Group 4 Group 5
 9:30 - 11:00 N3 N4 N9 N6 N2
11:00 - 11:20 coffee break
11:20 - 12:50 N4 N5 N1 N7 N9
  CLOSING REMARKS and lunch

Photogallery

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