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6th NIC@IIT Advanced Microscopy practical workshop

November 29 - December 3, 2021 at



  • Paolo Bianchini
  • Alberto Diaspro
Istituto Italiano di Tecnologia, Genova, Italy

Organizing committee

  • Paolo Bianchini
  • Alberto Diaspro
  • Manuela Salvatori
  • Ambra Giannetti
  • Andrea Chiappini

Link to all NIC@IIT Events

Supported by IIT, SIOF, SIBPA, Nikon

A limited number of students (approx. 30) will be admitted in order to have no more than 3 students for each instrument or set-up.

On November 29th, Lectures will be open access starting from 2,00 pm and ending at 7,00 pm. All together Farwell for invited speakers, students and instructors will be offered at 7,30 pm.

Topics discussed: Sample Preparation; Confocal Microscopy, Image Scanning Microscopy (ISM); STED; STORM, d-STORM; Live Cell Imaging; 2PEFM; FLIM; FCS; Fast Volumetric Imaging; Expansion Microscopy. Starting from November 30th we will have tutorials and practical sessions.

6th NIC@IIT school

Keynote Speakers and Lecturers

  • Loredana Casalis

  • Alberto Diaspro

  • Joerg Enderlein

  • Laura Finzi

  • Hans Gerritsen

  • Giancarlo Ruocco

  • Paolo Bianchini

  • Marco Castello

  • Irene Nepita

  • Simonluca Piazza

  • Colin Sheppard

  • Peter Saggau

  • Giuseppe Vicidomini

  • Isotta Cainero

  • Francesca Baldini

  • Eleonora Perego

  • Agnieszka M. Pierzynska-Mach

  • Eli Slenders

  • Giorgio Tortarolo

  • Alberta Trianni

  • Elena Cerutti

  • Salvatore Surdo

  • Alessandro Zunino

  • Chantal Usai

  • Matteo Mariangeli

  • Behjat S. Kariman

  • Francesco Garzella

  • Pietro Cirigliano

  • Michele Oneto

  • Mattia Pesce

  • Marco Scotto D'Abbusco

December 9 and 10, Technical Wrap-Up Meeting for Faculty only.


We would like to thank our partners for their continued support.

To inquire about becoming a partner please contact us.


For registration before performing the payment (link below) contact us:  email This email address is being protected from spambots. You need JavaScript enabled to view it. and This email address is being protected from spambots. You need JavaScript enabled to view it. with the mandatory subject "YOURSURNAME-6TH NIC at IIT-PRE". 

The first 10 registrations will receive a 50% discount!!!

Costo iscrizione/Fees

The fee will include the membership to SIOF, technical and scientific material, all together farewell buffet and lunches.

Topics and Workstations

The hands-on session will be organized to give basic knowledge on the following topics and the available instruments. The attendee will be divided into groups of a maximum of three people, and they will turn on all the workstations. While registering, please communicate which is your 2 or 3 most interesting workstations to learn about.


  • N1: Nikon confocal AX spectral resonant
    Pietro Cirigliano and Mattia Pesce

  • N2: Nikon N-STORM
    Agnieszka Pierzynska-Mach and Michele Oneto

  • N3: Nikon N-SIM
    Isotta Cainero and Elena Cerutti

  • N4: Nikon A1MP spectral resonant and FLIM (by ISS)
    Marco Scotto D'Abbusco and Behjat S. Kariman

  • N5Sample Preparation and Expansion Microscopy
    Francesca Baldini, Alberta Trianni and Chantal Usai

  • N6: Acousto-optic devices in microscopy and light sheet
    Salvatore Surdo, Alessandro Zunino and Francesco Garzella

  • N7: Custom ISM and spad-FFS
    Eli Slenders and Alessandro Rossetta

  • N8: Custom STED-ISM, FLISM
    Giorgio Tortarolo, Eleonora Perego and Matteo Mariangeli

  • N9: Genoa Instruments PRISM
    Marco Castello, Simonluca Piazza and Irene Nepita

  • N10: Lab tour and Q&A
    Alberto Diaspro, Peter Saggau and Paolo Bianchini


  • STORM (Stochastic Optical Reconstruction Microscopy), direct-STORM

  • SIM (Structured Illumination Microscopy)

  • Confocal Microscopy

  • ISM (Image Scanning Microscopy)

  • 2PEFM (Two-photon excitation fluorescence microscopy)

  • SHG (Second Harmonic Generation) microscopy

  • STED (STimulated Emission Depletion) microscopy

  • FLIM (Fuorescence Lifetime Imaging Microscopy) and FCS (Fluorescence correlation spectroscopy)

  • Label-Free microscopy

Day one, November 29

Keynote Lectures

  • Monday 29th November, 14.00 to 19.00
  • Location: Sala delle Grida - City Center - Piazza De Ferrari, Genova


14:00 - 14:15
Welcome and Opening remarks

Luigi Attanasio
President of the Chamber of Commerce in Genoa, Italy

14:15 - 14:30
Welcome and Opening remarks

Giorgio Metta
Scientific Director of Istituto Italiano di Tecnologia, Italy

14:30 - 14:45

Alberto Diaspro
Istituto Italiano di Tecnologia, Italy

14:45 - 15:30
Energy transfer and self quenching in fluorescence microscopy

Hans Gerritsen

University of Utrecht, Utrecht, Germany

15:30 - 16:15
Multiscale approaches to extracellular vesicle docking at cell membranes

Loredana Casalis

Elettra-Sincrotrone Trieste, Basovizza, Trieste, Italy

16.15 - 16:45
Coffee break
16:45 - 17:30
Mechanical properties imaging

Giancarlo Ruocco
Center for Life Nano & Neuro Science, Istituto Italiano di Tecnologia, Rome, Italy 

17:30 - 18:15
Single-molecule biophysics of transcription regulation

Laura Finzi
Department of Physics, Emory University, Atlanta, USA

18:15 - 19:00
Metal- and Graphene-Induced Energy Transfer Imaging

Joerg Enderlein
Institute of Physics – Biophysics, Georg August University, Göttingen, Germany

19:00 - 19:15
Closing remarks

Paolo Bianchini & Alberto Diaspro
Istituto Italiano di Tecnologia, Italy

Farewell, for registered students and speakers

Day two, November 30

  • Tuesday 30th November to Thursday 3rd December
  • Closed session for registered students.
  • Location: Istituto Italiano di Tecnologia, via E. Melen 83B (Great Campus Erzelli), Genova


Time12th Floor, GHT meeting room and virtual
9.00 - 9.45

Colin Sheppard, University of Wollongong, Australia
Confocal and image scanning microscopy

9:45 - 10.20

Peter Saggau, Baylor College of Medicine, USA
Acousto-optic devices in Microscopy

10.20 - 10.55

Giuseppe Vicidomini, Istituto Italiano di Tecnologia, Italy
Super-resolution laser scanning microscopy

10.55 - 11.30

Ranieri Bizzarri, University of Pisa, Italy
The pointillist approach, from single molecules to whole cells


Microscopy Labs, 9th floor
  • G#
    Group number
  • N#
    Workstation number
  G1  G2  G3  G4  G5  G6  G7   G8   G9
 14:15 - 15:15  N1  N2  N3 N4 N5 N6 N7 N8 N9
 15:20 - 16:20  N2  N3 N4 N5 N6 N7 N8 N9 N1
 16:40 - 17:40  N3  N4 N5 N6 N7 N8 N9 N1 N2
 17:45 - 18:45  N4 N5 N6 N7 N8 N9 N1 N2 N3


Day three, December 1

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8   G9
 9:30 - 10:30 N5 N6 N7 N8 N9 N1 N2 N3 N4
 10:45 - 11:45 N6 N7 N8 N9 N1 N2 N3 N4 N5
 12:00 - 13:00 N7 N8 N9 N1 N2 N3 N4 N5 N6
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 15:15 N8 N9 N1 N2 N3 N4 N5 N6 N7
15:20 - 16:20 N9 N1 N2 N3 N4 N5 N6 N7 N8
16:40 - 18:40 N9 N1 N2 N3 N4 N5 N6 N7 N8

Day four, December 2

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8   G9
 9:30 - 11:30 N8 N9 N1 N2 N3 N4 N5 N6 N10
 11:45 - 12:45 N7 N8 N9 N5 N6 N10 N4 N1 N2
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 16:15 N1 N7 N4 N10 N2 N3 N9 N5 N6
16:40 - 18:40 N5 N6 N3 N7 N10 N2 N8 N9 N1

Day five, December 3

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8   G9
 9:30 - 11:30 N6 N4 N8 N1 N9 N7 N5 N10 N3
 11:45 - 12:45 N3 N10 N10 N9 N1 N8 N2 N4 N5