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7th NIC@IIT Advanced Microscopy practical workshop

November 28 - December 2, 2022 at nic.iit.it

in memory of Prof. Tullio Pozzan

Info

download the flyer

Directors

  • Paolo Bianchini
  • Alberto Diaspro
Istituto Italiano di Tecnologia, Genova, Italy

Organizing committee

  • Paolo Bianchini
  • Alberto Diaspro
  • Manuela Salvatori
  • Ambra Giannetti
  • Alessandro Belardini

Link to all NIC@IIT Events

Link to all NIC@IIT Events

Supported by

IIT, SIOF, SIBPA, Nikon

PNRR Potenziamento Infrastrutture IR00023 SEE LIFE - Potenziamento Euro-BioImaging ERIC Italia

A limited number of students (approx. 50) will be admitted in order to have no more than 5 students for each instrument or set-up.

On November 28th, Lectures will be open access starting from 2,00 pm and ending at 7,00 pm. All together Farwell for invited speakers, students and instructors will be offered at 7,30 pm.

Topics discussed: Sample Preparation; Confocal Microscopy, Image Scanning Microscopy (ISM); STED; STORM, d-STORM; Live Cell Imaging; 2PEFM; FLIM; FCS; Fast Volumetric Imaging; Expansion Microscopy. Starting from November 30th we will have tutorials and practical sessions.

Genoa, Italy

6th NIC@IIT school

Keynote Speakers and Lecturers

  • Diego De Stefani

  • Timo Zimmermann

  • Anna Chiara De Luca

  • Spartaco Santi

  • Ranieri Bizzarri

  • Francesca Cella Zanacchi

  • Aymeric Le Gratiet

  • Giuseppe Vicidomini

  • Peter Saggau

  • Francesco Bacchi

  • Alberto Diaspro

  • Paolo Bianchini

  • Marco Castello

  • Simonluca Piazza

  • Irene Nepita

  • Davide Odino

  • Giacomo Cozzi

  • Eleonora Perego

  • Eli Slenders

  • Alessandro Zunino

  • Francesco Fersini

  • Sabrina Zappone

  • Andrea Bucci

  • Lisa Cuneo

  • Francesca Baldini

  • Isotta Cainero

  • Fabio Callegari

  • Chantal Usai

  • Matteo Mariangeli

  • Michele Oneto

  • Mattia Pesce

  • Marco Scotto D'Abbusco

Partners

We would like to thank our partners for their continued support.

Eurobioimaging
MOlecular-Scale Biophysics Research Infrastructure (MOSBRI)

To inquire about becoming a partner please contact us.

Contact us

Registration

For registration before performing the payment (link below) contact us:  email This email address is being protected from spambots. You need JavaScript enabled to view it. and This email address is being protected from spambots. You need JavaScript enabled to view it. with the mandatory subject "YOURSURNAME-7TH NIC at IIT-PRE". 

For IIT/SIOF/SIBPA/UNIGE members: 
PhD students and junior postdocs: 140 €
Postdocs: 220 €
groups (more than one person from the same research group): 100 € each person (both PhD students and postdocs)

7th NIC@IIT

Companies: 600 €
Non member:
PhD students and junior postdocs: 200 €
Postdocs: 270 €
before proceeding with the payment please contact us

The fee will include the membership to SIOF, technical and scientific material, all together farewell buffet and lunches.

Topics and Workstations

The hands-on session will be organized to give basic knowledge on the following topics and the available instruments. The attendee will be divided into groups of a maximum of three people, and they will turn on all the workstations. While registering, please communicate which is your 2 or 3 most interesting workstations to learn about.

Workstations

  • N1: Nikon confocal AX spectral resonant
    Giacomo Cozzi and Mattia Pesce

  • N2: Nikon N-STORM and N-SIM
    Isotta Cainero, Simone Civita and Lisa Cuneo

  • N3: Nikon A1MP spectral resonant and FLIM (by ISS)
    Marco Scotto D'Abbusco and Michele Oneto

  • N4: Nikon - CREST spinning disk V3
    Francesco Bacchi

  • N5: Sample Preparation and Expansion Microscopy
    Francesca Baldini and Chantal Usai

  • N6: Correlative fluorescence-atomic force microscopy
    Matteo Mariangeli and Davide Odino

  • N7: Custom ISM and spad-FFS
    Eli Slenders, Eleonora Perego and Andrea Bucci

  • N8: Custom STED-ISM, FLISM
    Alessandro Zunino, Francesco Fersini and Sabrina Zappone

  • N9: Genoa Instruments PRISM
    Marco Castello, Simonluca Piazza and Irene Nepita

  • N10: Polarization microscopy, Lab tour and Q&A
    Fabio Callegari, Alberto Diaspro, Peter Saggau, Ranieri Bizzarri, Spartaco Santi, Aymeric Le Gratiet and Paolo Bianchini

Topics

  • STORM (Stochastic Optical Reconstruction Microscopy), direct-STORM

  • SIM (Structured Illumination Microscopy)

  • Confocal Microscopy

  • ISM (Image Scanning Microscopy)

  • 2PEFM (Two-photon excitation fluorescence microscopy)

  • SHG (Second Harmonic Generation) microscopy

  • STED (STimulated Emission Depletion) microscopy

  • FLIM (Fuorescence Lifetime Imaging Microscopy) and FCS (Fluorescence correlation spectroscopy)

  • Expansion microscopy

  • Polarization microscopy

  • Label-Free microscopy

  • Correlative microscopy

Day one, November 28

Keynote Lectures

  • Monday 28th November, 14.00 to 19.00
  • Location: Hotel Bristol Palace, Via XX Settembre, 35, 16121 Genova

Program

TimeSpeaker/sTitle
14:00 - 14:05

Paolo Bianchini & Alberto Diaspro
Istituto Italiano di Tecnologia, Italy

Welcome and Opening remarks
14:05 - 14:45

Diego De Stefani
University of Padova, Padova, Italy

Imaging subcellular calcium dynamics
14:45 - 15:25

Timo Zimmermann
EMBL Imaging Centre, EMBL, Heidelberg, Germany

(Never) mind the gap. How to bring technology development closer to researchers at the EMBL Imaging Centre
15:25 - 16:05

Anna Chiara De Luca
IEOS-CNR, Napoli, Italy

Raman and SERS spectroscopy for cancer cell identification and imaging
16.05 - 16:30

coffee break

16:30 - 17.10

Ranieri Bizzarri
University of Pisa, Pisa, Italy

The pointillist approach, from single molecules to whole cells
17:10 - 17:50

Spartaco Santi
IGM-CNR, Bologna, Italy

Imaging of Extracellular Vesicles for Immunotherapy (confocal-STORM, TEM, STEM, AFM)
17:50 - 18:30

Francesca Cella Zanacchi
University of Pisa, Pisa, Italy

Single molecule localization microscopy goes quantitative
18:30 - 18.50

Francesco Bacchi
Crestoptics, Rome, Italy

Improvement in correlative microscopy: Nikon integration of CrestOptics spinning disk and SIM super-resolution modules
18.50 - 19:00

Paolo Bianchini & Alberto Diaspro
Istituto Italiano di Tecnologia, Italy

Closing remarks
19:30

Farewell, for registered students and speakers

Day two, November 29

  • Tuesday 29th November to Friday 2nd December
  • Closed session for registered students.
  • Location: Istituto Italiano di Tecnologia, via E. Melen 83B (Great Campus Erzelli), Genova

Program

Time10th Floor, Pitagora/Fibonacci meeting room and virtual
9:30 - 10.20

Peter Saggau, Baylor College of Medicine, USA
Acousto-optic devices in Microscopy

10.20 - 11.10

Aymeric Le Gratiet, ENSSAT, Lannion, France
Polarization-resolved optical microscopy: from micro- to macroscopic biological organisation

11.10 - 12.00

Giuseppe Vicidomini, Istituto Italiano di Tecnologia, Italy
Super-resolution laser scanning microscopy

12.00 - 12.20

Malik Soumare, Fluigent, France
Aria, automated system for multiplex imaging

Hands-on

Microscopy Labs, 9th floor

The students will be divided into groups (G#). All the groups will attend each workstation (N#) for at least an hour. After a complete turn, each group will focus on a subset of methods.
  • G#
    Group number
  • N#
    Workstation number
  G1 G2 G3 G4 G5 G6 G7 G8 G9
 14:15 - 15:15 N1 N2 N3 N4 N5 N6 N7 N8 N9
 15:20 - 16:20 N8 N3 N2 N5 N1 N7 N6 N9 N10
 16:40 - 17:40 N3 N4 N5 N2 N7 N8 N9 N10 N1
 17:45 - 18:45 N4 N5 N6 N7 N8 N9 N10 N2 N1

Day three, November 30

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8   G9
 9:30 - 10:30 N5 N6 N7 N8 N9 N1 N4 N2 N3
 10:40 - 11:40 N6 N7 N8 N10 N9 N2 N1 N3 N4
 11:45 - 12:45 N7 N8 N9 N1 N3 N6 N2 N4 N5
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 15:15 N2 N9 N10 N1 N4 N7 N3 N5 N6
15:20 - 16:20 N9 N2 N1 N8 N5 N4 N10 N6 N7
16:40 - 17:40 N3 N1 N2 N4 N6 N9 N5 N7 N8
17:45 - 18:45 N2 N1 N3 N5 N7 N10 N6 N8 N9

Day four, December 1

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8   G9
 9:30 - 10:30 N1 N5 N4 N10 N8 N3 N9 N6 N7
 10:45 - 12:45 N10 N8 N9 N3 N7 N5 N6 N1 N2
13:00 - 14:00 LUNCH (1st floor packet lunch reserved area)
 14:15 - 16:15 N9 N7 N4 N6 N2 N8 N1 N5 N10
16:40 - 18:40 N4 N6 N3 N7 N10 N2 N8 N9 N1

Day five, December 2

Hands on

  G1  G2  G3  G4  G5  G6  G7   G8   G9
 9:30 - 10:30 N6 N4 N8 N2 N9 N7 N5 N1 N3
 10:45 - 12:45 N3 N10 N7 N9 N6 N10 N2 N4 N5
  CLOSING REMARKS and lunch