5th NIC@IIT advanced practical workshop

December 3rd - December 6th, 2018 at nic.iit.it

Directors: Paolo Bianchini & Alberto Diaspro, Istituto Italiano di Tecnologia, Genova, Italy
  • A limited number of students (approx. 40) will be admitted in order to have no more than 3/4 students for each instrument or set-up.
  • On December 3rd, Lectures will be open access starting from 2,30 pm ending at 7,30 pm. All together dinner for invited speakers, students and instructors will be offered at 8 pm.
  • Basic courses will be included for students on:
    Confocal, Image scanning microscopy (ISM) and STED; STORM, d-STORM; Live cell imaging; 2-photon and FLIM/FRET; Fast volumetric imaging and particle tracking; Expansion Microscopy.
  • Starting from December 4th we will have tutorials and practical sessions.

Confirmed Faculty

  • Paolo Bianchini
  • Valentina Caorsi
  • Francesca Cella Zanacchi
  • Elisa d'Este
  • Alberto Diaspro
  • Marti Duocastella
  • Mario Faretta
  • Satoshi Kawata
  • Luca Lanzanò
  • Aymeric Le Gratiet
  • Daniel Lin
  • Danielle Love
  • Luca Pesce
  • Emiliano Ronzitti
  • Peter Saggau
  • Giorgio Tortarolo
  • Giuseppe Vicidomini

Partners

We would like to thank our partners for their continued support.
To inquire about becoming a partner please contact us.

Registration Fees

This email address is being protected from spambots. You need JavaScript enabled to view it., in order to book the seat or to apply for bursaries - payments can be done later - please, send an email to "This email address is being protected from spambots. You need JavaScript enabled to view it." and "This email address is being protected from spambots. You need JavaScript enabled to view it." with the mandatory subject "YOURSURNAME-5TH NIC at IIT-PRE". 

TypeFee in Euros (€)
PhD Student
200€
Post Doc
270€
Professionals or companies
600€
IIT/SIOF/SIBPA/UNIGE members: PhD students and junior Post Doc
140€
IIT/SIOF/SIBPA/UNIGE members: Post Docs
220€
The fee will include technical and scientific material, all together dinner and packet lunch.

Tutorials and courses

Each student should choose his favorite course and the corresponding group. We will try to satisfy all the requests. However, the maximum number of students per group is 4. Once assigned to a group, students cannot exchange them. Each group will attend eight "hands-on" lessons in eight different instruments as shown in the programme. The students, which bring their samples, could have the chance to image them.

Workstations

  • N1: Nikon multicolor 3D N-STORM
  • N2: IIT custom SPIM, IML-SPIM
  • N3: IIT custom gSTED, image scanning microscopy (ISM)
  • N4: Nikon N-SIM
  • N5: Nikon A1 spectral confocal
  • N6: Nikon Ti2 and Abbelight SAFe 360
  • N7: Fast Confocal Nikon spinning disk
  • N8: Genoa Instruments PRISM, image scanning microscopy
  • N9: Nikon A1R MP, ISS fast FLIM and FCS
  • N10: Expansion Microscopy

Tutorials

  • a: Nikon JOBS Mattia Pesce
  • b. Confocal, ISM Giuseppe Vicidomini
  • c. FLIM, FRET Luca Lanzanò
  • d. STED Giorgio Tortarolo
  • e. STORM, PALM, GSDIM Francesca Cella Zanacchi
  • f. SIM Giacomo Cozzi
  • g. Fast volumetric imaging Peter Saggau
  • h. Sample preparation for super-resolution microscopy Luca Pesce

Hands-on Courses

(all the students in groups (except G8) will have the chance to attend a session in each microscope)
Suggested Tutorials Hands-on (at least)
  • Confocal, ISM and STED
    b., d., h. N3, N5, N7, N8, N9
    Group number: G1, G2
  • STORM, d-STORM
    a., e., h. N1, N2, N4, N6, N7
    Group number: G3, G4
  • Live cell and fast volumetric imaging
    a., f., g., h. N2, N4, N6, N7, N9
    Group number: G5, G6
  • 2-photon and FLIM/FRET
    b., d., c., h. N2, N3, N5, N8, N9
    Group number: G7
  • Expansion Microscopy
    b., d. f., h. N4, N5, N7, N8, N10
    Group number: G8

The first day

Monday 3rd December, 09.30 to 19.00

Location: Sala delle Grida - City Center - Piazza De Ferrari, Genova

Program

Morning Tutorials

TimeMain RoomTelegrafo Room
09:30 - 10:30
a. (Time lapse)
b. (confocal)
10:30 - 11:30
e. (STORM)
d. (STED)
11:30 - 12:30
f. (SIM)
c. (FLIM, FRET)
12:30
Lunch Break

Afternoon - Keynote Lectures (Open access session)

TimeTitleSpeaker/s
14:00 - 14:15
Welcome and Opening remarks
Paolo Bianchini & Alberto Diaspro
Istituto Italiano di Tecnologia, Italy
14:15 - 15:15
Raman microscopy and plasmonics: label-free nano-imaging of biomolecules in a living cell
Satoshi Kawata
Osaka University, Japan
15:15 - 15:45
Into the nanoworld
Valentina Caorsi
Abbelight, Paris, France
15:45 - 16:15
LEDs: More than just a light sourceì
Danielle Love
Coolled, Andover, UK
16.15 - 16:45
Coffee break
16:45 - 17:30
Optical nanoscopy reveals unprecedented cytoskeletal features in the nervous system
Elisa d'Este
Max-Planck-Institute for Medical Research, Heidelberg, Germany
17:30 - 18:15
Neuronal circuits manipulation by wavefront shaping
Emiliano Ronzitti
Paris Descartes University, France
18:15 - 19:00
The Intelligent Microscope: a scalable resolution approach to high content and resolution imaging in cancer research
Mario Faretta
Department of Experimental Oncology European Institute of Oncology, Milan, Italy
19:00 - 20:00
Closing remarks
Alberto Diaspro
Istituto Italiano di Tecnologia, Italy
20:00
Social Dinner, for registered students and speakers

Hands-on

Tuesday 4th to Thursday 6th December 09.40 to 19.00

Please note that this is a closed session for registered students.

Location: Istituto Italiano di Tecnologia, via Morego 30, Genova

Tuesday 4th

TimeMontlacini room, IIT, Genova, Italy
09:40 - 10:20
g. (Fast volumetric imaging)
10:20 - 11:00
h. (Sample preparation)
11:00 - 11:40

Daniel Lin, Sunjinlab, Taiwan
RapiClear in Multiscale Images: from Macro to Micro

Hands-on

  G1 G2 G3 G4 G5 G6 G7 G8
11.45 - 12.45 N2 N4 N5 N8 N1 N3 N7 N10  (N9)
13.00 - 14..00 LUNCH
14.15 – 16.15 N3 N5 N9 N2 N7 N4 N8 N10 (N6)
16.45 – 18.45 N5 N7 N2 N4 N8 N6 N9 N10 (N3)

Wednesday 5th

  G1 G2 G3 G4 G5 G6 G7 G8
09.30 - 10.30 N6 N1 N3 N5 N9 N8 N4 N10 (N7)
10.45 - 12.45 N7 N8 N1 N6 N2 N9 N3 N10 (N5)
13.00 - 14.00 LUNCH
14.15 - 16.15 N8 N9 N6 N7 N4 N2 N5 N10 (N1)
16.45 - 18.45 N9 N3 N4 N1 N6 N7 N2 N10 (N8)

Thursday 6th

  G1 G2 G3 G4 G5 G6 G7 G8
09.30 - 10.30 N1 N6 N7 N9 N3 N5 N8 N10 (N2)
10.45 - 11.45 N4 N2 N8 N3 N5 N1 N6 N10 (N9)
12.00 - 13.00 N8 N9 N6 N4 N7 N2 N1 N10 (N5)
13.00 - 14.00 LUNCH
14.15 - 16.15 N3 N8 N7 N1 N9 N4 N5 N10 (N6)
16.45 - 18.45 N5 N3 N1 N6 N4 N7 N9 N10 (N2)
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