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5th NIC@IIT advanced practical workshop/school

December 3rd - December 6th, 2018 at nic.iit.it

school directors: Paolo Bianchini & Alberto Diaspro, Istituto Italiano di Tecnologia, Genova, Italy

A limited number of students (approx. 40) will be admitted in order to have no more than 3/4 students for each instrument or set-up. 

On December 3rd, Lectures will be open access starting from 2,30 pm ending at 7,30 pm. All together dinner for invited speakers, students and instructors will be offered at 8 pm.

Basic courses will be included for students on: 

Confocal, Image scanning microscopy (ISM) and STED; STORM, d-STORM; Live cell imaging; 2-photon and FLIM/FRET; Fast volumetric imaging and particle tracking; Expansion Microscopy.

Starting from December 4th we will have tutorials and practical sessions. 

 registration fees 

 keynote lectures 

 programme of the courses 


Confirmed Faculty

  • Paolo Bianchini
  • Valentina Caorsi
  • Francesca Cella Zanacchi
  • Elisa d'Este
  • Alberto Diaspro
  • Marti Duocastella
  • Mario Faretta
  • Satoshi Kawata
  • Luca Lanzanò
  • Aymeric Le Gratiet
  • Daniel Lin
  • Luca Pesce
  • Emiliano Ronzitti
  • Peter Saggau
  • Giorgio Tortarolo
  • Giuseppe Vicidomini

Registration Fees

For registration,  

This email address is being protected from spambots. You need JavaScript enabled to view it., in order to book the seat or to apply for bursaries - payments can be done later - please, send an email to "This email address is being protected from spambots. You need JavaScript enabled to view it." and "This email address is being protected from spambots. You need JavaScript enabled to view it." with the mandatory subject "YOURSURNAME-5TH NIC at IIT-PRE". 

TypeFee in Euros (€)
PhD student 200€
Post Doc 270€
Professionals or companies 600€
IIT/SIOF/SIBPA/UNIGE members: PhD students and junior Post Doc 140€
IIT/SIOF/SIBPA/UNIGE members: Post Docs 220€
The fee will include technical and scientific material, all together dinner and packet lunch. 

The first Day

Monday 3rd December, 09.30 to 19.00

Location: Sala delle Grida - City Center - Piazza De Ferrari, Genova


    1. Nikon JOBS Mattia Pesce
    2. Spinning disk / Confocal, ISM Giuseppe Vicidomini
    3. Multiphoton, FLIM, FRET Luca Lanzanò
    4. STED, STED-FCS Giorgio Tortarolo
    5. STORM, PALM, GSDIM Francesca Cella Zanacchi
    6. SIM Giacomo Cozzi
  Main room Telegrafo room
09.30 – 10.30  a. (Time lapse) b. (confocal)
10.30 – 11.30  e. (STORM) d. (STED)
11.30 – 12.30 f. (SIM) c. (FLIM, FRET) 

12.30 Lunch break

Keynote Lectures

Open access session
14.00 Paolo Bianchini & Alberto Diaspro, Istituto Italiano di Tecnologia, Italy
Welcome and Opening remarks
14.15 Satoshi Kawata
15.15 Valentina Caorsi 
16.00 coffee break
16.30 Elisa d'Este, Max-Planck-Institute for Medical Research, Heidelberg, Germany
Optical nanoscopy reveals unprecedented cytoskeletal features in the nervous system
17.15 Emiliano Ronzitti 
18.00 Mario Faretta
18.45 Alberto Diaspro, Istituto Italiano di Tecnologia, Italy
Closing remarks
20.00 Social Dinner, for registered students and speakers

Hands On

Tutorials and Practical workshop

Please note that this is a closed session for registered students.

Location: Istituto Italiano di Tecnologia, via Morego 30, Genova

Each student should choose his favorite course and the corresponding group. We will try to satisfy all the requests. However, the maximum number of students per group is 4. Once assigned to a group, students cannot exchange them. Each group will attend eight "hands on" lessons in eight different instruments as shown in the programme.


    1. Fast volumetric imaging Marti Duocastella
    2. Sample preparation for super-resolution microscopy  Luca Pesce


  Suggested Tutorials Hands on (at least) Group number
Confocal, ISM and STED b., d., h. N3, N5, N6, N9, N10 G1, G2
STORM, d-STORM a., e., h. N1, N2, N5, N7, N8 G3, G4
Live cell imaging a., f., h. N2, N4, N5, N7, N8 G5
2-photon and FLIM/FRET b., d., c., h. N2, N3, N5, N6, N10 G6
Fast volumetric imaging and particle tracking.    a., c., g. N1, N2, N7, N5, N11 G7
Expansion Microscopy b., d. f., h. N4, N8, N9, N5, N12 G8


N1: Nikon multicolor 3D N-STORM
N7: Fast Confocal Nikon spinning disk
N8: IIT custom 2C 3D pulsed STED
N3: IIT custom gSTED, CARMA-Gi
N9: Nikon A1R MP, ISS fast FLIM and FCS
N4: Nikon N-SIM
N10: single particle tracking
N5: Nikon A1 spectral confocal N11: Expansion Microscopy
N6: Nikon Time lapse  

Tuesday 4th

  HACK room, IIT, Genova, Italy  
10.00 – 11.00 h. (Sample preparation)  
 11.00 – 12.00 g. (Fast volumetric imagingSample preparation)  
   G1   G2   G3   G4   G5   G6   G7   G8 
14.00 – 16.00 N9  N1  N4  N7  N2  N5 N6 N11
16.30 – 18.30                

Wednesday 5th

  G1 G2 G3 G4 G5 G6 G7 G8
09.30 – 10.30                
11.30 – 12.30 N8 N5 N10 N4  N3 N2  N7 N1
12.30 – 14.00 LUNCH
14.00 – 16.00 N2 N9 N8 N10 N1 N6 N4 N11
16.30 – 18.30 N7 N8 N6 N9 N10 N1 N2 N4 

Thursday 6th

  G1 G2 G3 G4 G5 G6 G7 G8
09.30- 10.30                
10.30 – 11.30 N4  N7  N2 N5  N6 N9  N1 N8 
12.00 – 14.00 LUNCH
14.00 – 16.00 N3  N4  N1 N6 N7 N8  N10 N5 
16.30 – 18.30 N5 N3  N7 N1 N4  N10 N9 N11

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