5th NIC@IIT advanced practical workshop

December 3rd - December 6th, 2018 at nic.iit.it

Directors: Paolo Bianchini & Alberto Diaspro, Istituto Italiano di Tecnologia, Genova, Italy

A limited number of students (approx. 40) will be admitted in order to have no more than 3/4 students for each instrument or set-up.
On December 3rd, Lectures will be open access starting from 2,30 pm ending at 7,30 pm. All together dinner for invited speakers, students and instructors will be offered at 8 pm.
Basic courses will be included for students on:
Confocal, Image scanning microscopy (ISM) and STED; STORM, d-STORM; Live cell imaging; 2-photon and FLIM/FRET; Fast volumetric imaging and particle tracking; Expansion Microscopy.
Starting from December 4th we will have tutorials and practical sessions.

Confirmed Faculty

Paolo Bianchini
Valentina Caorsi
Francesca Cella Zanacchi
Elisa d'Este
Alberto Diaspro

Marti Duocastella
Mario Faretta
Satoshi Kawata
Luca Lanzanò

Aymeric Le Gratiet
Daniel Lin
Danielle Love
Luca Pesce

Emiliano Ronzitti
Peter Saggau
Giorgio Tortarolo
Giuseppe Vicidomini

Partners

We would like to thank our partners for their continued support.

To inquire about becoming a partner please contact us.

Registration Fees

This email address is being protected from spambots. You need JavaScript enabled to view it., in order to book the seat or to apply for bursaries - payments can be done later - please, send an email to "This email address is being protected from spambots. You need JavaScript enabled to view it." and "This email address is being protected from spambots. You need JavaScript enabled to view it." with the mandatory subject "YOURSURNAME-5TH NIC at IIT-PRE".

Type	Fee in Euros (€)
PhD Student	200€
Post Doc	270€
Professionals or companies	600€
IIT/SIOF/SIBPA/UNIGE members: PhD students and junior Post Doc	140€
IIT/SIOF/SIBPA/UNIGE members: Post Docs	220€

The fee will include technical and scientific material, all together dinner and packet lunch.

Tutorials and courses

Each student should choose his favorite course and the corresponding group. We will try to satisfy all the requests. However, the maximum number of students per group is 4. Once assigned to a group, students cannot exchange them. Each group will attend eight "hands-on" lessons in eight different instruments as shown in the programme. The students, which bring their samples, could have the chance to image them.

Workstations

N1: Nikon multicolor 3D N-STORM
N2: IIT custom SPIM, IML-SPIM
N3: IIT custom gSTED, image scanning microscopy (ISM)
N4: Nikon N-SIM
N5: Nikon A1 spectral confocal
N6: Nikon Ti2 and Abbelight SAFe 360
N7: Fast Confocal Nikon spinning disk
N8: Genoa Instruments PRISM, image scanning microscopy
N9: Nikon A1R MP, ISS fast FLIM and FCS
N10: Expansion Microscopy

Tutorials

a: Nikon JOBS Mattia Pesce
b. Confocal, ISM Giuseppe Vicidomini
c. FLIM, FRET Luca Lanzanò
d. STED Giorgio Tortarolo
e. STORM, PALM, GSDIM Francesca Cella Zanacchi
f. SIM Giacomo Cozzi
g. Fast volumetric imaging Peter Saggau
h. Sample preparation for super-resolution microscopy Luca Pesce

Hands-on Courses

(all the students in groups (except G8) will have the chance to attend a session in each microscope)

The first day

Monday 3rd December, 09.30 to 19.00

Location: Sala delle Grida - City Center - Piazza De Ferrari, Genova

Program

Morning Tutorials

Time	Main Room	Telegrafo Room
09:30 - 10:30	a. (Time lapse)	b. (confocal)
10:30 - 11:30	e. (STORM)	d. (STED)
11:30 - 12:30	f. (SIM)	c. (FLIM, FRET)
12:30	Lunch Break

Afternoon - Keynote Lectures (Open access session)

Time	Title	Speaker/s
14:00 - 14:15	Welcome and Opening remarks	Paolo Bianchini & Alberto Diaspro Istituto Italiano di Tecnologia, Italy
14:15 - 15:15	Raman microscopy and plasmonics: label-free nano-imaging of biomolecules in a living cell	Satoshi Kawata Osaka University, Japan
15:15 - 15:45	Into the nanoworld	Valentina Caorsi Abbelight, Paris, France
15:45 - 16:15	LEDs: More than just a light sourceì	Danielle Love Coolled, Andover, UK
16.15 - 16:45	Coffee break
16:45 - 17:30	Optical nanoscopy reveals unprecedented cytoskeletal features in the nervous system	Elisa d'Este Max-Planck-Institute for Medical Research, Heidelberg, Germany
17:30 - 18:15	Neuronal circuits manipulation by wavefront shaping	Emiliano Ronzitti Paris Descartes University, France
18:15 - 19:00	The Intelligent Microscope: a scalable resolution approach to high content and resolution imaging in cancer research	Mario Faretta Department of Experimental Oncology European Institute of Oncology, Milan, Italy
19:00 - 20:00	Closing remarks	Alberto Diaspro Istituto Italiano di Tecnologia, Italy
20:00	Social Dinner, for registered students and speakers

Hands-on

Tuesday 4th to Thursday 6th December 09.40 to 19.00

Please note that this is a closed session for registered students.

Location: Istituto Italiano di Tecnologia, via Morego 30, Genova

Tuesday 4th

Time	Montlacini room, IIT, Genova, Italy
09:40 - 10:20	g. (Fast volumetric imaging)
10:20 - 11:00	h. (Sample preparation)
11:00 - 11:40	Daniel Lin, Sunjinlab, Taiwan RapiClear in Multiscale Images: from Macro to Micro

Hands-on

	G1	G2	G3	G4	G5	G6	G7	G8
11.45 - 12.45	N2	N4	N5	N8	N1	N3	N7	N10 (N9)
13.00 - 14..00	LUNCH
14.15 – 16.15	N3	N5	N9	N2	N7	N4	N8	N10 (N6)
16.45 – 18.45	N5	N7	N2	N4	N8	N6	N9	N10 (N3)

Wednesday 5th

	G1	G2	G3	G4	G5	G6	G7	G8
09.30 - 10.30	N6	N1	N3	N5	N9	N8	N4	N10 (N7)
10.45 - 12.45	N7	N8	N1	N6	N2	N9	N3	N10 (N5)
13.00 - 14.00	LUNCH
14.15 - 16.15	N8	N9	N6	N7	N4	N2	N5	N10 (N1)
16.45 - 18.45	N9	N3	N4	N1	N6	N7	N2	N10 (N8)

Thursday 6th

	G1	G2	G3	G4	G5	G6	G7	G8
09.30 - 10.30	N1	N6	N7	N9	N3	N5	N8	N10 (N2)
10.45 - 11.45	N4	N2	N8	N3	N5	N1	N6	N10 (N9)
12.00 - 13.00	N8	N9	N6	N4	N7	N2	N1	N10 (N5)
13.00 - 14.00	LUNCH
14.15 - 16.15	N3	N8	N7	N1	N9	N4	N5	N10 (N6)
16.45 - 18.45	N5	N3	N1	N6	N4	N7	N9	N10 (N2)